• Test Kits

  • Premixed • Premeasured • Precise

Oxygen (dissolved) Test Kits

Oxygen (Dissolved) Test Kits

Visual Kits

Range MDL Method Kit Catalog No. Refill Catalog No.
0-20 ppb 2 ppb Rhodazine D K-7511 R-7511
0-40 ppb 2.5 ppb Rhodazine D K-7540 R-7540
0-100 ppb 5 ppb Rhodazine D K-7599 R-7540
5-180 ppb 5 ppb Rhodazine D K-7518 R-7518
0-1 ppm 0.025 ppm Rhodazine D K-7501 R-7501
1-12 ppm 1 ppm Indigo Carmine K-7512 R-7512

Instrumental Kits

Range Method Kit Catalog No.
0-1.000 ppm Rhodazine D K-7553
0-15.0 ppm Indigo Carmine K-7513
0-15.0 ppm Indigo Carmine I-2002


The level of dissolved oxygen in natural waters is often a direct indication of quality, since aquatic plants produce oxygen, while microorganisms generally consume it as they feed on pollutants. At low temperatures the solubility of oxygen is increased; during summer, saturation levels can be as low as 4 ppm. Dissolved oxygen (D.O.) is essential for the support of fish and other aquatic life and aids in the natural decomposition of organic matter. Waste treatment plants that employ aerobic digestion must maintain a level of at least 2 ppm dissolved oxygen.

At elevated temperatures, oxygen is highly corrosive to metals, causing pitting in systems such as high-pressure boilers and deep well oil recovery equipment. To prevent costly corrosion damage, the liquids in contact with the metal surfaces must be treated to mitigate oxygen levels in water, usually by a combination of physical and chemical means. Deaeration can reduce the dissolved oxygen concentration of boiler feedwater from several ppm to a few ppb. Chemical reducing agents such as hydrazine, DEHA, or sodium sulfite, may be used instead of or in conjunction with deaeration.

The Indigo Carmine Method

References: ASTM D 888-87, Dissolved Oxygen in Water, Test Method A. Gilbert, T. W., Behymer, T. D., Castañeda, H. B., “Determination of Dissolved Oxygen in Natural and Wastewaters,” American Laboratory, March 1982, pp. 119-134.
Dissolved Oxygen test kits for environmental and drinking water applications (ppm range) employ the indigo carmine method. The reduced form of indigo carmine reacts with D.O. to form a blue product. The indigo carmine methodology is not subject to interferences from temperature, salinity, or dissolved gases such as sulfide, which plague users of D.O. meters. Results are expressed as ppm (mg/L) O2.
The Rhodazine D™ Method

References: Developed by CHEMetrics, LLC ASTM Power Plant Manual, 1st. ed. p. 169 (1984). ASTM D 5543-15, Low Level Dissolved Oxygen in Water. Department of the Navy, Final Report of NAVSECPHILADIV Project A – 1598, Evaluation of CHEMetrics Feedwater Dissolved Oxygen Test Kit (1975).
Test kits for boiler waters and applications requiring trace levels of D.O. (ppb range) employ the Rhodazine D methodology. Developed by CHEMetrics, LLC, and approved by ASTM as the reference method for ppb D.O. determination, the Rhodazine D compound in reduced form reacts with dissolved oxygen to form a bright pink reaction product. The method is not subject to salinity or dissolved gas interferences. Oxidizing agents, including benzoquinone, can cause high results. Reducing agents such as hydrazine and sulfite do not interfere. Results are expressed as ppm (mg/L) or ppb (ug/L) O2.Low-range dissolved oxygen test kits include a special sampling tube for use with boiler feedwater. This device allows the user to break the tip of the ampoule in a flowing sample stream in order to preclude error from contamination by atmospheric oxygen.

Application Overview:  Monitoring Dissolved Oxygen in High-Pressure Boiler Systems

Spanish Version